Isolation of components and hypoglycemic effect of methanolic basil extract (ocium basilicum) in C57bl/6 rats with experimental hyperglycemia
DOI:
https://doi.org/10.51481/amc.v59i2.957Keywords:
Mellitus diabetes, hyperglycemia, rats, hypoglycemic, natural medicine, pharmacologyAbstract
Background: Basil (Ocium basilicum) is an herb belonging to the family laminaceae, characterized by its medicinal benefits. Its use has been referred for the treatment of cancer, vitiligo, hypercholesterolemia, and diabetes mellitus.
Objective: to evaluate the hypoglycemic effect of the methanol extract of basil, isolation and purification of its main compounds. Methods: The leaves and stems were collected in the sector La Hechicera, Mérida, Venezuela State. Samples were macerated in 20L of methanol to 70% v/v, dosing to increaseddoses between 1.0 and2.0 g/kg, using as experimental model, male rats of the C57BL/6 strain with hyperglycemia induced with alloxan monohydrate. A positivecontrol was included using as a hypoglycemic agent sitagliptin (400µg/kg). The extract was submitted to fractionation by open column chromatography,, whose fractions (175 ml each) were partnered by structural similarity and were dosed to the population under study. Serial blood samples from the base of the tail vein were obtained and were processed using the method of the glucose oxidase-peroxidase.
Results: they showed a decrease of the concentration of blood glucose at a dose of 2.0 g/kg (< 120 mg/dL). Structural analyses were conducted using chromatographic, spectroscopic, spectrometric and chemical tests. They were isolated from the study and the following compounds were identified: nandecilato of methyl (C20O2 H40); methyl behenate (C23O2H46), methyl hexacosanoate (C27O2H54) as well as 18-methoxycarbonyl-3, 4-didehidroibogamina, 5,7,3´-trihydroxi-3,6,4´- trimetoxiflavona flavonoid, which are reported for the first time in the phytochemical analysis for O. basilicum.
Conclusion: These findings support the potential use of Basil as a weed of traditional medicine in the hypoglycemic treatment.
Downloads
References
Wittman P. Das gro?e Buch der Kráuter und Gewürze - Basilikum. Teub. 2008; 138: 219 - 227.
Werker E.. Function of essential oil secreting glandular hairs in aromatic plants of the Lamiaceae: a review. Flavour and Flagrance Journal. 1993; 8: 249-255.
Chirinos M, Velásquez R, Ascanio C, Mata J, Carrasqueño A. Obtención de los aceites esenciales de la albahaca (Ocimum basilicum) a partir de tejidos cultivados in vivo e in vitro.Rev. Fac. Agron. UCV. 2009; 35: 28-33.
Lezama K, Pereyra S, Limo S. Lochroma smithianum (Solanaceae) una nueva especie del Departamento La Libertad, Perú. Arnaldoa 2008;14: 23 -28.
Amarowicz R, Karamac M, Shahidi F. Antioxidant activity of phenolic fractions of lentil (Lens culinaris). Journal of Food Lipids. 2003; 10: 1-10.
Rosero A, Camacho R, Polanco M, Gómez S. Efecto relajante de las hojas de Ocimum basilicum y Foeniculum vulgare colombianas en íleon aislado de rata. Univ Med Bogotá 2009; 58: 98-109.
Colivet J, Belloso G, Hurtado E. Comparative study of the inhibiting effects of sweet pepper (Capsicum chinense) extracts on the growth of Escherichia coli AND Bacillus sp. Saber. 2006; 18:168-173.
Chiang L, Ng L, Cheng P, Chiang W, Lin, C. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clin Exp Pharmacol Physiol. 2005; 32: 811-6.
González J, González H, González S, Rosales T, Andrade I. Microextracción en fase sólida de compuestos volátiles en albahaca (Ocimu basilicum L.). Act Univ Guanaja. 2011; 21: 17-23.
Carretero ME. Compuestos fenólicos: Taninos, Pano. Actual. Med. 2011; 235:633-636.
Meléndez M, Alvarado S. Antihyperglycaemic effect of the rue (Ruta graveolens) methanol extract in an experimental model of hyperglycemic rats. Rev Fac Cs Vets UCV. 2011; 52: 119-126.
Manual sobre el cuidado y uso de animales de experimentación. Consejo Canadiense de Protección de los Animales.1998. Recuperado el 02 de enero de 2016. Disponible en: http://www.fcv.unl.edu.ar/comite/ ManualsobreelcuidadoyusodeanimalesdeexperimentaionConsejoCanadiense
Declaración de Helsinki. 2008. Principios éticos para las investigaciones médicas. Asamblea Médica Mundial, Helsinki, Finlandia.
Figueroa L, Díaz F, Camacho A, López M. Efectos inducidos por Cnidoscolus chayamansa Mac Vaugh y Citrus aurantium L. sobre los niveles de glucosa, colesterol y triacilglicéridos en un modelo de rata diabética. Rev. Bras. Farmacogn., 2009; 19:898-907.
Fleitas A, Carballo R, Almeida G, Quintela A, Alfonso, M. Modelo experimental de diabetes en conejos. Rev. Cubana. Angiol. Circ. Vasc., 2000; 1:10-14.
Loyola V. Biosíntesis de los alcaloides indólicos. Una revisión crítica. Rev Soc Quím de México. 2004;48: 67-94.
Virkow J, Meléndez M. 2011. Stimulatory effect of pancreatic b-cells of rabbit p-cymene compound isolated and purified from wild plants. 2nd International Congress 2011 on Analytical Proteomics. 18 -21 July. Madrid, Spain.
Dreikorn K. The role of phytotherapy in treating lower urinary tract symptoms and benign prostatic hyperplasia. World J. Urol. 2002; 19:426-435.
Dreikorn K, Berges R, Pientka L, Jonas U. Phytotherapy of benign prostatic hyperplasia. Current evidence-based evaluation. Urologe. 2002; 41:447-451
Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomed. 1995; 2: 137-189.
Carvalheira J, Zecchin H, Saad M. Vias de sinalização da insulina. Arq. Bras. Endocrinol. Metab. 2002; 46:419-425.
Di Girolamo G, Tamez A, Tamez H. Inhibidores de la dipeptidil peptidasa-4:
farmacodinamia, farmacocinética y seguridad. Med. Int. Mex. 2008; 24:142-7
Arroyo J, Martínez J, Ronceros G, Palomino R, Villarreal A, Bonilla P. Coadjuvant hypoglycemiant effect of Annona muricata L (guanábana) leaves ethanolic extract in patients with type 2 diabetes mellitus receiving glibenclamide treatment. An. Fac. Med. 2009;70: 163-167
Feinstein D, Spagnol A, Akar C, Weinberg G, Murphy P, Gavrilyuk V, Dello C. Receptor-independent actions of PPAR thiazolidinedione agonists: Is mitochondrial function the key?. Biochem. Pharmac. 2005; 70: 177-188.
Bailey C. Potential new treatments for type 2 diabetes. Tren. Pharmac. Scien. 2000; 21:259-265.
Panunti B, Fonseca V. Mechanisms and therapeutic targets in type 2 diabetes mellitus Drug Discovery Today. Dises. Mechan. 2000; 1:151-157.
Downloads
Published
Versions
- 2017-05-09 (2)
- 2017-05-09 (1)
Issue
Section
License
Copyright (c) 2017 Acta Médica Costarricense
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Los autores que publican en la revista Acta Médica Costarricense pueden distribuir, copiar, remezclar, retocar, leer, descargar, imprimir, buscar y crear a partir de su obra de modo no comercial, indicando los créditos a la revista y sus autores y compartir su obra en las mismas condiciones. Para ello se aplica la licencia Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional(CC BY-NC-SA 4.0)
