Peripheral blood stem cell collection by apheresis
DOI:
https://doi.org/10.51481/amc.v56i2.836Keywords:
stem cells, CD34 cells, apheresis, collectionAbstract
Background: To evaluate performance and quality of peripheral blood stem cell collections performed at the México Hospital from June 2010 to June 2012, since there is an established minimum dose of CD34+ cells that determines the probability of success of engraftment.Methods: A retrospective study using patient records and data obtained from peripheral blood stem cell apheresis procedures carried out at the Haematology Unit of the México Hospital. This information was used to calculate performance and quality values of collections, and the effect of these on the recovery time of haematological parameters in patients transplanted between June 2010 and June 2012.
Results: A total of 52 peripheral blood stem cell apheresis procedures were performed to 26 patients (Hodgkin lymphoma 46%, non-Hodgkin lymphoma and multiple myeloma 27%). An average of 8 blood volumes per patient was processed, the median collection was 8.48 x108 mononuclear cells/Kg, of which an average of 3.06 x106/Kg were CD34+ cells. The average recovery time of haematological parameters after transplant was 15.05 days for leukocytes and 17.15 days for platelets. An inverse correlation was observed between CD34+ infused cell count and time for platelet count recovery, but not for leukocyte count recovery.
Conclusions: Cell counts obtained from apheresis collections and recovery time of haematological parameters after transplant were consistent with results described by the medical literature, showing that the protocol followed has been successful. Only leukocyte count previous to apheresis has proved to be useful to predict a quality collection, and CD34+ infused cell count is the best parameter to predict a successful engraftment.
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References
Cooling L, Hoffmann S, Herrst M, Muck C, Armelagos H, Davenport R. A prospective randomized trial of two popular mononuclear cell collection sets for autologous peripherical blood stem cell collection in multiple myeloma. Transfusion 2010; 50: 100-119.
Aguilar D, Lordmendez D. Recolección de células progenitoras en niños. Rev Mex Med Tran, 2010; 3, Supl. 1: S71-S74.
Tempescul A, Ianotto J.C, Hardy E, Quivoron F, Petrov L, Berthou C. Peripheral blood stem cell collection in elderly patients. Ann Hematol 2010; 89: 317-321.
Akhtar S, Weshi A, Rahal M, Khafaga Y, Tbakhi a, Humaidan H, maghfoor I. Factors affecting autologous peripheral blood stem cell collection in patients with relapsed or refractory diffuse large cell lymphoma and Hodking lymphoma: a single institution results of 168 patients. Leuk Lymphoma 2008; 49:769-778.
Lopez F. Recolección de células progenitoras hematopoyéticas mediante aféresis de grandes volúmenes. Hemasferio 2012: 22-26.
Moreb J.S, Salmasinia D, Hsu J, Hou W, Cline C, Rosenau E. Long-TermOutcome after Autologous StemCell Transplantation with Adequate Peripheral Blood Stem Cell Mobilization Using Plerixafor and G-CSF in PoorMobilizer Lymphoma and Myeloma Patients. Adv hematol 2011, Article ID 517561, 8 pages doi:10.1155/2011/517561.
Wang S, Nademanee A, Qian D, Dagis A, Park H-S, Fridey J, et al. Peripheral blood hematopoietic stem cell mobilization and collection efficacy is not an independent prognostic factor for autologous stem cell transplantation. Transfusion 2007; 47: 2207-2216.
Hoggatt J, Pelus L.M. Mobilization of hematopoietic stem cells from the bone marrow niche to the blood compartment. Stem Cell Res Ther 2011: 2-13.
Awan F.T, Kochuparambil S.T , DeRemer D, Cumpston
A, Craig M, Jillella A, Hamadani M. Plerixafor Salvage Is
Safe and Effective in Hard-to-Mobilize Patients Undergoing
Chemotherapy and Filgrastim-Based Peripheral Blood Progenitor Cell Mobilization. J Oncol 2012, Article ID 931071, 5 pages doi:10.1155/2012/931071.
Vantelon J.M, Koscielny S. Brault P, Bourhis J.H, Ribrag V, Pico J, et al. Scoring system for the prediction of successful peripheral blood stem cell (PBSC) collection in non.Hodgkin´s lymphoma (NHL): application in clinical practice. Bone Marrow Transplant 2000; 25: 495-499.
Subair A. C, Grant r, Wu W, Tun H, Rivera C, Moreno-Aspitia A, et al. Platelet count is a sensitive predictor of autologous peripheral blood progenitor cell collection yield in previously treated plasma cell disease patients. Transfusion 2008; 48: 1106-1114.
Ford C.D, Chan K.J, Reilly W.F, Petersen F.B. An evaluation of predictive factors for CD34+ cell harvest yields from patients mobilized with chemotherapy and growth factors. Transfusion 2003; 43: 622-625.
Figuerres E, Haut P.R, Olzewski M, Kletzel M. Analysis of parameters affecting engraftment in children undergoing autologous peripherical blood stem cell transplants. Bone Marrow Transplant 2000; 24: 583-588.
Kim J.e, Yoo C, Kim S, Lee D.H, Kim S, Lee J.S, Suh C. Optimal timing of G-CSF administration for effective autologous stem cell collection. Bone Marrow Trasplant 2011; 46: 806-812.
Weaver C.H, Hazelton B, Birch R, Palmer P, Allen C, Schwartzberg L, West W. An analysis of engraftment kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy. Blood 1995; 86: 3961-3969.
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