Immunoglobulin G anti-helicobacter pylori by ELISA and Western-Blot in patients of the Gastroenterology Service of Hospital San Vicente de Paul, Heredia
DOI:
https://doi.org/10.51481/amc.v42i1.538Keywords:
H. pylori, IgG, ELISA, Western-blot, sueroAbstract
Background and aim: In Costa Rica the gastric cancer is the malignancy that produces more deaths. In Other studies their pathology it has been associated with Helicobacter pylori. This bacteria is able to produce substances that alter the gastric mucosa, antigens that have been caracterized by Westernblot. The purpose of this study was to performed for the first time in our country a study of IgG-anti H. pylori by Westernblot to identify the antigens that induce an immune response, using sera and strains of H. pylori isolated from Costa Rican patients. Also the results obtained by Western-blot were compared with an ELISA test to determine the clinical sensibility and specificity using the gastric biopsy as reference.
Method: Samples consisted of gastric biopsies and sera from 83 patients refered to the Endoscopy Service of the San Vicente de Paul Hospital, Heredia. To each patient a histologic study of gastric biopsy was realized and antibodies study of IgG anti H. pylori by ELISA and Western-blot.
Results and conclutions: In the Western-blot protein bands of difTerent molecular weight with a predominance of H. pyIori antigens of Iow molecular weight were observed. The Western-blot and ELISA had a clinical sensitivity of and 88% and a clinical specificity of 73% and 71% respectively. when compared with the histology.
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