Analysis of twelve polymorphic markers in the DNA of a population sample from the Costa Rican Central Valley
DOI:
https://doi.org/10.51481/amc.v41i3.516Keywords:
micro y minisatélites del ADN, PCR de polimorfismos humanos, identificación humanaAbstract
Aim: To establish databases of allele frequencies in a Costa Rican Central Valley population sample.
Methods: Peripheral blood samples from more than 40 individuals were used to isolate DNA and analyze each simple with 10 dinucleotide repeat genetic markers and with 2 minisatellite repeats, using the polymerase chain reaction.
Alleles were identified by comparision with DNA from CEPH family members. Genotypes were determined by labelling one of the two PCR primers with 32 P before amplification, electrophoresis in sequencing gels and autoradiography.
Results and Conclusions: Analysis of this data set indicates that this sample is in Hardy-Weinberg equilibrium and shows no evidence of linkage disequilibrium between markers. These data are compared with results from other human populations analyzed with the same markers, finding similarities in allele frequencies among them. Notably, the Costa Rican simple presents the lowest heterozygosity value, with 4 of the 10 dinucleotide markers tested, followed by a Cerdenian sample. In contrast, the two African samples presented the highest heterozygosity indexes with a larger number of alleles.
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