Prevalence of microsporidia in urine of patients referred for urine culture in the Central Pacific Region

Abstract

Urine samples from patients with clinical antecedents of sus-picion genitourinary pathology were studied for bacteria,fungi and for the presence of microsporidia. Usual culturemedia and methods were used to study bacteria and fungiorganisms. To study for microsporidian parasites, nine mlwere centrifuged and smears of the sediment were fixed, pre-vious tratment with 5% KOH and then stained according toa modified Weber method.Groups of Microsporidia spores were foud in twenty five percent of the samples, coming more frenquently from peoplebetween 26 to 55 five years old .Since these parasites cause important diseases, especially inimmunosuppressed human beings, but also in immunocom-petent persons, it is necessary to improve the methods for abetter diagnosis of this parasitosis. Some modifications havebeen done to the Weber technique in order to have the bestresults.